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KMID : 0942820030020020080
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Journal of Korean Brain Tumor Society
2003 Volume.2 No. 2 p.80 ~ p.85
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Inhibition of Telomerase Activity from Brain Tumors
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Park Dong-June
Kang Soo-Kyung
Cha Seung-Heon
Choi Chang-Hwa
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Abstract
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Objective: Telomerase activity was examined in brain tumor tissues and the effects of several catechins on telomerase activity were studied.
Methods: Telomerase activity was examined in brain tumor tissues with the TRAP assay and compared with histological diagnosis and the CD68 proliferative cell index(PCI). The effects of several catechins and cisplatin on telomerase activity were studied in a cell-free system. And the effects of several catechins and cisplatin on growth rate were determined using two brain cancer cell lines, SNB19 and T98G human glioblastoma.
Results: Telomerase assay revealed telomerase activity in all four glioblastomas, six of seven anaplastic astrocytomas, one of three astrocytomas, all cases of an anaplastic oligodendroglioma, an oligodendroglioma, an anaplastic meningioma, a melanoma and a medulloblastoma, all three metastatic brain tumors, two of six schwannomas and all two craniopharyngiomas. There was no correlation between telomerase activity and CD68 PCI. Telomerase was strongly inhibited by epicatechin gallate(ECG(-)), epigallocatechin gallate(EGCG(-)) and cisplatin at the concentrations of 1mM, 1mM, 20mM respectively. A limitation on the continued growth of two representative brain cancer cell lines, in the presence of 1mM ECG(-) and EGCG(-), was observed.
Conclusion: The activation of telomerase is associated with the potential tumor growth in malignant brain tumors. In addition, therapeutic implication of anti-telomerase approach in brain tumors were indicated.
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KEYWORD
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Telomerase, Brain tumor, Telomerase inhibitor
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